IonQuant is a label free quantification tool for timsTOF PASEF DDA and non-timsTOF (e.g., Orbitrap) data.
- Fast
- Accurate
- Java 1.8+.
ext
folder from MSFragger.
The latest IonQuant stanalone JAR can be downloaded from here.
java -jar IonQuant.jar <options> <.d/.mzML/.mzXML/.raw/.pepXML/_quant.csv files>
Options:
--threads <integer> # Number of threads. 0 = all logical cores. Default: 0
--mztol <float> # MS1 tolerance in PPM. Default: 10.0
--imtol <float> # 1/K0 tolerance. Default: 0.05
--rttol <float> # Retention time tolerance. Unit: min. Default: 0.4
--seedmz 0/1 # M/Z used as the start point of tracing. 0 = calculated M/Z; 1 = observed M/Z. Default: 0
--minisotopes 1/2/3 # Required isotopes in feature extraction. Default: 2
--psm <string> # Path to Philosopher's psm.tsv. One --psm indicates one psm.tsv and can have multiple --psm. Optional. Default: <blank>
--multidir <string> # Output directory for the multi experimental result. Optional. Default: <blank>
--normalization 0/1 # Normalize the intensities across all runs. Default: 1
--minions <integer> # Minimum ions required in quantifying proteins. Default: 2
--proteinquant 1/2 # Protein quantification algorithm. 1 = top-N, 2 = MaxLFQ. Default: 1
--minfreq <float> # Minimum required frequency of an ion being selected for protein quantification. Default: 0.5
--tp <int> # Number of ions used in quantifying each protein. If 0, using all ions. Default: 3
--mbr 0/1 # Perform match-between-runs. Default: 0
--mbrrttol <float> # Retention time tolerance used in match-between-runs. Unit: min. Default: 1.0
--mbrimtol <float> # 1/K0 tolerance used in match-between-runs. Default: 0.05
--mbrtoprun <integer> # Maximum number of matched runs in mapping one run. Default: 3
--mbrmincorr <float> # Minimum required correlation coefficient in matching two runs. Default: 0.5
--ionmobility 0/1 # The data has ion mobility information or not (for conventional LC-MS data). Default: 0
--ionfdr <float> # Transferred ion false discovery rate threshold. Default: 0.01
--peptidefdr <float> # Transferred peptide false discovery rate threshold. Default: 0.01
--proteinfdr <float> # Transferred protein false discovery rate threshold. Default: 0.01
--label <string> # A pair of light and heavy labelling mass. Can have multiple --label for multiple pairs of light and heavy labelling, but one peptide can only have one of these pairs. Format: <light mass>;<heavy mass>. Optional. Default: <blank>
--requantify 0/1 # Re-quantify unidentified feature based on identified feature. Only activate when --label is not empty. Default: 0
Note: in some high-performance computing (HPC) servers, you may need to explicitly specify --threads <integer>
in case that Java cannot correctly get the logical core number.
Fast quantitative analysis of timsTOF PASEF data with MSFragger and IonQuant