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Hi my bioconda install is reporting this needs Py 3.0. I can set up a Py 2.7 environment for just this of course, but wondered if there would be a Py3.6+ version or if I am being silly in some way? Kind ...
  • RichardBJ
  • Opened 
    on Nov 1, 2022
  • #45

Hi, what is the principle of AfterQC to detect bubbles? how is it reflected in the data?
  • yinbinqiu
  • Opened 
    on Sep 29, 2022
  • #44

Hi! I managed to run AfterQC succesfully the first time I used it with one of my libraries with the following command line: pypy /home/pop_manuel/proyecto_transcriptoma_rhizophagus/software/AfterQC-master/after.py ...
  • mquijano1
  • 1
  • Opened 
    on Jan 12, 2022
  • #43

It may be a good idea to implement and option to remove specific sequences like overrepresented sequences, usually being rRNA or PCR artefacts; well passing these sequences in a fasta or identifying them ...
  • manuelsmendoza
  • Opened 
    on Aug 20, 2020
  • #42

Hi there I am getting the following error Traceback (most recent call last): File /Users/apple/miniconda3/envs/py27/bin/after.py , line 228, in module main() File /Users/apple/miniconda3/envs/py27/bin/after.py ...
  • sankadinesh
  • Opened 
    on May 31, 2020
  • #41

I would like to ask a question. Can I parse paired-end files after the process joined by AfterQC?
  • fabianomenegidio
  • Opened 
    on Jan 29, 2019
  • #39

Hi, Shifu I and my colleague (@yodeng) encountered this issue recently. This issue may be caused by the reassignment of empty list to self.records at a href= https://github.com/OpenGene/AfterQC/blob/master/circledetector.py#L19 ...
  • slw287r
  • Opened 
    on Nov 28, 2018
  • #38

AfterQC is constantly counting wrong the total bases in paired end Miseq fastq reads. For example two paired end files with total bases 611,060,153 (as calculated by various programs) it seems to have ...
  • pbravakos
  • 1
  • Opened 
    on Jul 4, 2018
  • #37

Hi , in preprocesser.py line 498 , your code is : if lowQual1 self.options.unqualified_base_limit or lowQual1 self.options.unqualified_base_limit: I suppose the code should be : if lowQual1 self.options.unqualified_base_limit ...
  • emojinchuan
  • Opened 
    on Jun 19, 2018
  • #36

Hello, I have using afterqc with default settings to remove adapter sequences from RNA Seq read files. My original files have all the reads of uniform length. After processing with afterqc the sequence ...
  • coolmak32
  • 7
  • Opened 
    on Mar 9, 2018
  • #35
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