- New
AddAzimuthScores()
andAddAzimuthResults()
functions - Add
shuffle
parameter toFeatureScatter()
(#4280) - Add
lsiproject
andrpca
options forFindTransferAnchors()
- Add
rlsi
option forFindIntegrationAnchors()
- Preserve feature metadata when converting from
SingleCellExperiment
toSeuratObject
class (#4205) - Preserve multiple assays when converting from
SingleCellExperiment
toSeuratObject
class (#3764) - Fix passing of
score.thresh
parameter inScoreJackStraw()
(#4268) - Fix FC calculation in
FindMarkers()
non-log transformed data. - Add umap-learn version >= 0.5.0 compatibility for
RunUMAP()
- Fix
DotPlot
to uselog1p
whenscale=False
(#4298) - Fix split and shuffled
DimPlot
- Disallow NULL or another length 0 vector for
ident.1
inFindMarkers()
- Fix range shift when labeling clusters on a GeomSpatial plot
- Fix SpatialPlot distortion for non-square images.
- Fix future-related warnings in
FindIntegrationAnchors()
- Fix
fc.name
parameter inFindMarkers()
(#4474) - Deprecate
group.by
parameter inPlotPerturbScore()
in favor ofmixscape.class
.
- Add direction option to
PlotClusterTree()
- Add
cols
parameter toJackStrawPlot()
- Add
ReadMtx()
to read local and remote mtx files with associated cell and feature name files
- Equality added to differential expression thresholds in
FindMarkers
(e.g, >= logfc.threshold rather than >) Read10X()
now prepends dataset number for first dataset when reading multiple datasets- Bug fix for
subset.AnchorSet()
- Bug fix for fold change values in
FindMarkers()
when setting a different pseudocount (#4111) - Bug fix for
RunLDA()
related to proper passing of assay parameter. - When using
order=TRUE
inSingleDimPlot()
, print NA points under all others. - Remove default parameter value for
data.dir
inRead10X()
- Import spatstat fxns from subpackages (spatstat.core, spatstat.geom)
RunUMAP
now checks for graph/neighbor consistency
- Expose
FoldChange()
component inFindMarkers()
. - Add the
merge.DimReduc
method - Add
IntegrateEmbeddings()
to correct embeddings ofDimReduc
s - Add
ProjectUMAP()
to project query cells into a reference UMAP space - Add
MapQuery()
as a wrapper aroundIntegrateData()
,IntegrateEmbeddings()
, andProjectUMAP()
- Add
MappingScore
to compute a per-cell mapping score used in Azimuth - Add
AggregateExpression()
for summation based pseudobulk calculations - Add mixscape functionality via
CalcPerturbSig()
,PrepLDA()
,RunLDA()
,DEenrichRPlot()
,MixscapeHeatmap()
,MixscapeLDA()
,PlotPerturbScore()
,RunMixscape()
- Add
FindSubCluster()
to further cluster existing clusters - Add supervised PCA functionality via
RunSPCA()
- Add functionality to enable weighted nearest neighbor analyses via
FindMultiModalNeighbors()
- Add neighbor visualization plot via
NNPlot()
. - Add
PredictAssay()
to impute expression or embeddings from nearest neighbors - Add
Graphs()
function to access the names of the stored Graph objects or pull a specific one - Add checks for NA, NaN, logical, non-integer, and infinite values during CreateAssayObject and NormalizeData.default
- Add
AnnotateAnchors()
to aid in AnchorSet interpretation as well assubset.AnchorSet()
- Add flexibility of choice for cell column in
Read10X()
- Add rasterization option to
FeatureScatter()
andVariableFeaturePlot()
- Add step1 feature parameters in the SCTModel via
PrepVSTResults()
- Default neighbor finding algorithm changed from "rann" to "annoy"
- Default
ncells
parameter inSCTransform()
changed to 5000 - Default fold change in
FindMarkers()
changed from ln to log2 - Implementation improvements to
AverageExpression()
AnchorSet
class re-implemented as a virtual class from whichIntegrationAnchorSet
andTransferAnchorSet
now inherit.- Point size in
VlnPlot()
now set automatically if not specified - Return the sample.tree properly when integrating with a single reference dataset
- Replace
as.character.quosure
usage withas_label
due to deprecation - Minor changes to the exact calculation of the anchor weight matrix
- Default rasterization limit in
DimPlot()
andFeaturePlot()
changed from 50,000 to 100,000 SCTransform()
now returns a formalizedAssay
subclassSCTAssay()
- When using
normalization.method='SCT'
inFindTransferAnchors()
, normalize query using reference SCT model when possible. - Change default Neighbor name in
FindNeighbors
toAssay.nn
CreateGeneActivityMatrix
replaced bySignac::GeneActivity()
RunLSI
replaced by bySignac::RunTFIDF()
andSignac::RunSVD()
ReadAlevin
andReadAlevinCsv
moved to SeuratWrappersExportToCellbrowser
andStopCellbrowser
moved to SeuratWrappers
- Titles added to
DimPlot
when specifyinggroup.by
parameter keep.scale
parameter added toFeaturePlot
to control scaling across multiple features and/or splits.
Same
deprecated in favor ofbase::identity
- Fix in
DietSeurat
to work with specializedAssay
objects - Fix p-value return when using the
ape
implementation of Moran's I - Fix bug in FindMarkers when using MAST with a latent variable
- Updates to
Key<-.DimReduc
that allow handling of empty reduction column names - Allow setting
ctrl
inCellCycleScoring
- Modify subset.Seurat to allow specialized Assay subsetting methods
- Fix image selection in interactive spatial plots
- Update Rcpp functions with
export(rng=FALSE)
to avoid potential future warnings - Fix RenameCells bug for integrated SCT assays
- Fix highlight order with proper factor levels when using
SetHighlight
in plots - Small change in CellRanger version detection logic of h5 file to improve robustness to outside tools.
do.cpp
deprecated and will default to true
- Set the seed in
WhichCells
regardless of whether or notidents
is passed - Retain Graph and Neighbor objects when subsetting only on features
- Fix data.frame input to
CreateAssayObject()
when data.frame has no rownames. - Default annoy search to sequential if not using multicore future plans.
- Require sctransform >= 0.3.0
- Added support for nearest neighbor input and
return.model
parameter inRunUMAP()
- Enable named color vectors in
DoHeatmap()
- Add
label.color
andlabel.box
parameters toDimPlot
- Added
shuffle
andseed
parameters toDimPlot()
to help with overplotting - Added new stacked violin plot functionality
- Allow setting
slot
parameter inRunUMAP
- Added support for FIt-SNE v1.2+
- Fix for
Spatial*Plot
when running with interactive=TRUE - Set max for number of items returned by
Top
and remove duplicate items when balanced=TRUE - Fix logging bug when functions were run via
do.call()
- Fix handling of weight.by.var parameter when approx=FALSE in
RunPCA()
- Fix issue where feature names with dashes crashed
CellSelector
- Fix issue where errors in subsetting were being swallowed
- Fix issue where labeling uncropped spatial plots was broken
CreateActivityMatrix
deprecated in favor ofSignac::GeneActivity
ReadAlevin
andReadAlevinCsv
deprecated in favor ofSeuratWrappers::ReadAlevin
ExportToCellbrowser
andStopCellbrowser
deprecated in favor ofSeuratWrappers::ExportToCellbrowser
andSeuratWrappers::StopCellbrowser
ReadH5AD
andWriteH5AD
deprecated in favor of h5Seurat/H5AD functionality found in SeuratDiskas.loom
andas.Seurat.loom
deprecated in favor of functionality found in SeuratDisk
- Added ability to create a Seurat object from an existing Assay object, or any object inheriting from the Assay class
- Added ability to cluster idents and group features in
DotPlot
- Added ability to use RColorBrewer plaettes for split
DotPlots
- Added visualization and analysis functionality for spatially resolved datasets (Visium, Slide-seq).
- Removed
add.iter
parameter fromRunTSNE
function - Fixed integer overflow error in the WilcoxDETest function
- Minor visual fixes in
DoHeatmap
group bar + labels - Efficiency improvements in anchor scoring (
ScoreAnchors
) - Fix bug in
FindClusters()
when the last node has no edges - Default to weighted = TRUE when constructing igraph objects in
RunLeiden
. Remove corresponding weights parameter fromFindClusters()
. - Fix handling of keys in
FeatureScatter()
- Change
CellSelector
to use Shiny gadgets instead of SDMTools - Mark
PointLocator
as defunct - Remove
SDMTools
- Fixed data slot return in
AverageExpression
when subsetting features and returning a Seurat object
- New
scale
parameter inDotPlot
- New
keep.sparse parameter in
CreateGeneActivityMatrix` for a more memory efficient option - Added ability to store model learned by UMAP and project new data
- New
strip.suffix
option inRead10X
. This changes the default behavior ofRead10X
. A trailing-1
present in all cell names will not be removed by default. - Added
group.by
parameter toFeatureScatter
- Replace wilcox.test with limma implementation for a faster FindMarkers default method
- Better point separation for
VlnPlot
s when using thesplit.by
option - Efficiency improvements for anchor pairing
- Deprecate redundant
sort.cell
parameter inFeaturePlot
- Fixes to ensure correct class of Matrix passed to c++ functions
- Fixes for underscores in ident labels for
DotPlot
- Ensure preservation of matrix dimnames in
SampleUMI
- Fix non-standard evaluation problems in
subset
andWhichCells
- Default split violin option is now a multi group option
- Preserve alpha in
FeaturePlot
when usingblend
- Update
assay.used
slot forDimReduc
s when Assay is renamed
- Fixes to
DoHeatmap
to remain compatible with ggplot2 v3.3 - Adoption of
patchwork
framework to replaceCombinePlots
- New system agnostic
Which
function to address problems with FItSNE on Windows
- Export
CellsByIdentities
andRowMergeSparseMatrices
functions - nCount and nFeature metadata variables retained after subset and updated properly with
UpdateSeuratObject
- Fix uwot support for running directly on feature matrices
- Fixes for keys with underscores
- Fix issue with leiden option for
FindClusters
- Fix for data transfer when using sctransform
- SDMTools moved to Suggests as package is orphaned
- New silent slot updater
- New random seed options to
RunCCA
,RunTSNE
,WhichCells
,HTODemux
,AddModuleScore
,VlnPlot
, andRidgePlot
- Enhancements for dealing with
Assay
-derived objects
- Only run
CalcN
(generates nFeatures and nCounts) whencounts
changes - Fix issue regarding colons in feature names
- Change object class testing to use
inherits
oris.*
for R 4.0 compatability
- New
RegroupIdents
function to reassign idents based on metadata column majority UpdateSymbolList
function to pull new gene names from HGNC- Added support for H5AD layers as additional assays in a
Seurat
object
- Fix rownames issue when running UMAP on dist object
- Add support for new H5AD
obsm
andvarm
stucture - Fix issue when trying to read non-existent feature-level metadata from an H5AD file
- Fix in integration workflow when using SCTransform
- Improved error checking for
AddModuleScore
- cbind fix in reference-based integration (
MapQuery
) - Fix for convenience plots error hanging
- Ensure Seurat objects aren't stored in the command logs
- New
PrepSCTIntegration
function to facilitate integration afterSCTransform
- Reference-based integration with the
reference
parameter inFindIntegrationAnchors
- Reciprocal PCA as a
reduction
option inFindIntegrationAnchors
- New
CollapseEmbeddingOutliers
function - Enable
FindTransferAnchors
afterSCTransform
- Added back
ColorDimSplit
functionality - Include a code of conduct
- Added uwot support as new default UMAP method
- Added
CheckDots
to catch unused parameters and suggest updated names Reductions
andAssays
assays functions to list stored DimReducs and Assays
- Fix regex in
LogSeuratCommand
- Check for NAs in feature names in
Read10X
- Prevent dimnames for counts/data/scale.data matrices from being arrays
- Updates
ReadH5AD
to distinguish FVF methods - Fixes to UpdateSeuratObject for v2 objects
- Sink all output from stdout to stderr
- Fix to scale.data cell ordering after subsetting
- Enable
Assay
specification inBuildClusterTree
- Fix
FeaturePlot
when using bothblend
andsplit.by
- Fix to
WhichCells
when passingcells
andinvert
- Fix to
HoverLocator
labels and title - Ensure features names don't contain pipes (
|
) - Deprecation of
RunLSI
andRunALRA
- Fix legend bug when sorting in
ExIPlot
- Flag to skip singleton grouping in
FindClusters
- New custom colors for blended
FeaturePlot
s - New
GetResidual
function - New Seurat/Monocle converters
- Fix issue where certain assays weren't being shown in the
Seurat
object - Fix issue where we weren't updating
DimReduc
object column names - Fix line spacers in
DoHeatmap
- Fix uninformative labels in
FeaturePlot
- Fix unset identities when converting from SCE to Seurat
- Fix single colors being interpreted as palettes in
SingleDimPlot
- Ensure factor levels are always numerically increasing after
FindClusters
- Better cell highlighting colors for
DimPlot
- Fix to
levels<-.Seurat
- Add ability to use counts/scaled data in
BuildClusterTree
- Minor fix to split
ScaleData
- Add global option (Seurat.memsafe) to skip gc() calls
- Restore draw.lines to DoHeatmap, maintain size of color bar with different number of features (#1429)
- Enable split.by parameter for ScaleData
- Add slot parameter to FeaturePlot (#1483)
- Add assay parameter to DotPlot (#1404)
- Fix to color options for VlnPlot with split.by option (#1425)
- Improvements to conversion functions (loom, SCE)
- Fix for cluster tree reordering (#1434)
- Fix PercentageFeatureSet for single feature case
- Fix to fold change calculation and filtering for other slots in FindMarkers (#1454)
- Keep title vectorized in AugmentPlot (#1515)
- Export LogSeuratCommand function
- Fix for FindConservedMarkers when one ident is missing from a group (#1517)
- New method for identifying anchors across single-cell datasets
- Parallelization support via future
- Additional method for demultiplexing with MULTIseqDemux
- Support normalization via sctransform
- New option for clustering with the Leiden algorithm
- Support for reading 10X v3 files
- New function to export Seurat objects for the UCSC cell browser
- Support for data import from Alevin outputs
- Imputation of dropped out values via ALRA
- Significant code restructuring
- Most occurances of "gene(s)" in function names/arguments renamed to "feature(s)"
- Changes to the Seurat object class to facilitate multimodal data
- New BlendPlot implementation
- GetIdent function added to pull identity info
- DiffusionMap dependency replaced with destiny to avoid archival
- Java dependency removed and functionality rewritten in Rcpp
- Speed and efficiency improvements for Rcpp code
- More robust duplicate handling in CellCycleScoring
- New HTOHeatmap function
- Support for custom PNG arguments for vector-friendly plotting
- Fix for 'NA'-labeled cells disappearing with custom color scale
- Replaced FNN with RANN
- Removed unused compiler flags
- Moved several lightly-used packages from 'imports' to 'suggests'
- RenameCells added for easy renaming of all cells
- Read10X_h5 added to read in 10X formatted h5 files
- SetAssayData ensures cell order is the same between assay objects and the Seurat object
- Compatability updates for ggplot2 v2.3.0
- Support for UMAP dimensional reduction technique
- New conversion functions for SingleCellExperiment and anndata
- FetchData preserves cell order
- Require Matrix 1.2-14 or higher
- AddModuleScore no longer densifies sparse-matrices
- Various visualization fixes and improvements
- Default value for latent.vars in FindMarkers/FindAllMarkers changed to NULL.
- Support for HTO demultiplexing
- Utility functions: TransferIdent, CombineIdent, SplitObject, vector.friendly
- C++ implementation for parts of BuildSNN
- Preliminary parallelization support (regression and JackStraw)
- Support for FItSNE
- MetaDE replaced with metap for combining p-values (MetaDE was removed from CRAN)
- NMF heatmaps replaced (NMF to be archived by CRAN)
- MetaDE replaced with metap for combining p-values (MetaDE was removed from CRAN)
- NMF heatmaps replaced (NMF to be archived by CRAN)
- Multiple alignment functionality with RunMultiCCA and AlignSubspace extended to multiple datasets
- CalcAlignmentScore added to evaluate alignment quality
- MetageneBicorPlot added to guide CC selection
- Change cluster order in DoHeatmap with group.order parameter
- Ability to change plotting order and add a title to DimPlot
- do.clean and subset.raw options for SubsetData
- JoyPlot has been replaced with RidgePlot
- FindClusters is now more robust in making temp files
- MetaDE support for combining p-values in DE testing
- Support for using MAST and DESeq2 packages for differential expression testing in FindMarkers
- Support for multi-modal single-cell data via @assay slot
- Default DE test changed to Wilcoxon rank sum test
- Now available on CRAN
- Updated documentation complete with examples
- Example datasets:
pbmc_small
andcc.genes
- C++ implementation for parts of FindVariableGenes
- Minor bug fixes
- New method for aligning scRNA-seq datasets
- Significant code restructuring
- New methods for scoring gene expression and cell-cycle phases
- New visualization features (do.hover, do.identify)