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Documents related to tetrode electrophysiology for neural data recording

and a few more general tutorials

Will attempt to store here useful tutorials and other documents related to electrophysiology using tetrodes.

Table of contents:

  1. HOW-TO: use Markdown to format readme (.md) files on GitHub: markdown tutorial
  2. HOW-TO: goldplating for 12 microns Nichrome wire. Mostly from John Bladon in the Jadhav lab. 4_gold_plating_Nichrome_12um
  3. HOW-TO: do electrolytic lesions to mark the tips of tetrodes for histology, better for 17 um diameter wire (pdf)
    • let me know if you'd like to update it with parameters of your choice!
    • a better method especially for smaller wire, this can mark the tetrode tracks as well (e.g. 12.7 Nichrome): better lesion method
  4. HOW-TO: slice a frozen brain with a microtome: microtome_slicing
  5. HOW-TO: stain mounted slices with Nissl staining (Cresyl Violet): nissl_staining
  6. Collaborative hyperdrive parameters list (google spreadsheet)
    • feel free to contribute by adding a row!
  7. How to do a successful untethered recoring with SpikeGadgets (128ch headstage): tutorial
  8. Drive-building tutorial for Axona, rat, single-bundle drives ('poor-lady'). Credits mostly to Roddy Grieves: tutorial
  9. Tetrode-making tutorial (tape method): tetrode tutorial

Feel free to use & share and add comments as issues here or on Mastodon at https://neuromatch.social/@elduvelle_neuro

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