diff --git a/pyfluo/data.py b/pyfluo/data.py
index d39c41d..2de4796 100644
--- a/pyfluo/data.py
+++ b/pyfluo/data.py
@@ -1434,4 +1434,16 @@ def process_roi(self, dist_thresh=5, **kwargs):
             closeby_kw.update(distance_thresh = dist_thresh_pix)
 
         roi_new = process_roi(roi, dff, closeby_kw=closeby_kw, **kwargs)
+        
+        # remove anything outside motion borders
+        y0,x0 = int(np.floor(self.motion_borders.ymin)), int(np.floor(self.motion_borders.xmin))
+        y1,x1 = int(np.ceil(self.motion_borders.ymax)), int(np.ceil(self.motion_borders.xmax))
+        if roi_new.ndim == 2:
+            roi_new = np.array([roi_new])
+        roi_new = roi_new.astype(bool)
+        roi_new[:,:y0,:] = False
+        roi_new[:,y1:,:] = False
+        roi_new[:,:,:x0] = False
+        roi_new[:,:,x1:] = False
+
         self.set_roi(roi_new)