diff --git a/bwamem.c b/bwamem.c index 38ab6e6b..f7809492 100644 --- a/bwamem.c +++ b/bwamem.c @@ -607,113 +607,6 @@ static inline int infer_bw(int l1, int l2, int score, int a, int q, int r) return w; } -void bwa_hit2sam(kstring_t *str, const int8_t mat[25], int q, int r, int w, const bntseq_t *bns, const uint8_t *pac, bseq1_t *s, const bwahit_t *p_, int is_hard, const bwahit_t *m) -{ -#define is_mapped(x) ((x)->rb >= 0 && (x)->rb < (x)->re && (x)->re <= bns->l_pac<<1) - int score, n_cigar, is_rev = 0, rid, mid, copy_mate = 0, NM = -1; - uint32_t *cigar = 0; - int64_t pos = -1; - bwahit_t ptmp, *p = &ptmp; - - if (!p_) { // in this case, generate an unmapped alignment - memset(&ptmp, 0, sizeof(bwahit_t)); - ptmp.rb = ptmp.re = -1; - } else ptmp = *p_; - p->flag |= m? 1 : 0; // is paired in sequencing - p->flag |= !is_mapped(p)? 4 : 0; // is mapped - p->flag |= m && !is_mapped(m)? 8 : 0; // is mate mapped - if (m && !is_mapped(p) && is_mapped(m)) { - p->rb = m->rb; p->re = m->re; p->qb = 0; p->qe = s->l_seq; - copy_mate = 1; - } - p->flag |= p->rb >= bns->l_pac? 0x10 : 0; // is reverse strand - p->flag |= m && m->rb >= bns->l_pac? 0x20 : 0; // is mate on reverse strand - if (is_mapped(p) && m && !is_mapped(m) && (p->flag&0x10)) p->flag |= 0x20; // if mate is unmapped, it takes the strand of the current read - kputs(s->name, str); kputc('\t', str); - if (is_mapped(p)) { // has a coordinate, no matter whether it is mapped or copied from the mate - int sam_flag = p->flag&0xff; // the flag that will be outputed to SAM; it is not always the same as p->flag - if (p->flag&0x10000) sam_flag |= 0x100; - if (!copy_mate) { - int w2; - w2 = infer_bw(p->qe - p->qb, p->re - p->rb, p->score, mat[0], q, r); - w2 = w2 < w? w2 : w; - cigar = bwa_gen_cigar(mat, q, r, w2, bns->l_pac, pac, p->qe - p->qb, (uint8_t*)&s->seq[p->qb], p->rb, p->re, &score, &n_cigar, &NM); - p->flag |= n_cigar == 0? 4 : 0; // FIXME: check why this may happen (this has already happened) - } else n_cigar = 0, cigar = 0; - pos = bns_depos(bns, p->rb < bns->l_pac? p->rb : p->re - 1, &is_rev); - bns_cnt_ambi(bns, pos, p->re - p->rb, &rid); - kputw(sam_flag, str); kputc('\t', str); - kputs(bns->anns[rid].name, str); kputc('\t', str); kputuw(pos - bns->anns[rid].offset + 1, str); kputc('\t', str); - kputw(p->qual, str); kputc('\t', str); - if (n_cigar) { - int i, clip5, clip3; - clip5 = is_rev? s->l_seq - p->qe : p->qb; - clip3 = is_rev? p->qb : s->l_seq - p->qe; - if (clip5) { kputw(clip5, str); kputc("SH"[(is_hard!=0)], str); } - for (i = 0; i < n_cigar; ++i) { - kputw(cigar[i]>>4, str); kputc("MIDSH"[cigar[i]&0xf], str); - } - if (clip3) { kputw(clip3, str); kputc("SH"[(is_hard!=0)], str); } - } else kputc('*', str); - } else { // no coordinate - kputw(p->flag, str); - kputs("\t*\t0\t0\t*", str); - rid = -1; - } - if (m && is_mapped(m)) { // then print mate pos and isize - pos = bns_depos(bns, m->rb < bns->l_pac? m->rb : m->re - 1, &is_rev); - bns_cnt_ambi(bns, pos, m->re - m->rb, &mid); - kputc('\t', str); - if (mid == rid) kputc('=', str); - else kputs(bns->anns[mid].name, str); - kputc('\t', str); kputuw(pos - bns->anns[mid].offset + 1, str); - kputc('\t', str); - if (mid == rid) { - int64_t p0 = p->rb < bns->l_pac? p->rb : (bns->l_pac<<1) - 1 - p->rb; - int64_t p1 = m->rb < bns->l_pac? m->rb : (bns->l_pac<<1) - 1 - m->rb; - kputw(-(p0 - p1 + (p0 > p1? 1 : p0 < p1? -1 : 0)), str); - } else kputw(0, str); - kputc('\t', str); - } else if (m && is_mapped(p)) { // then copy the position - kputsn("\t=\t", 3, str); - kputuw(pos - bns->anns[rid].offset + 1, str); - kputsn("\t0\t", 3, str); - } else kputsn("\t*\t0\t0\t", 7, str); - if (p->flag&0x100) { // for secondary alignments, don't write SEQ and QUAL - kputsn("*\t*", 3, str); - } else if (!(p->flag&0x10)) { // print SEQ and QUAL, the forward strand - int i, qb = 0, qe = s->l_seq; - if (!(p->flag&4) && is_hard) qb = p->qb, qe = p->qe; - ks_resize(str, str->l + (qe - qb) + 1); - for (i = qb; i < qe; ++i) str->s[str->l++] = "ACGTN"[(int)s->seq[i]]; - kputc('\t', str); - if (s->qual) { // printf qual - ks_resize(str, str->l + (qe - qb) + 1); - for (i = qb; i < qe; ++i) str->s[str->l++] = s->qual[i]; - str->s[str->l] = 0; - } else kputc('*', str); - } else { // the reverse strand - int i, qb = 0, qe = s->l_seq; - if (!(p->flag&4) && is_hard) qb = p->qb, qe = p->qe; - ks_resize(str, str->l + (qe - qb) + 1); - for (i = qe-1; i >= qb; --i) str->s[str->l++] = "TGCAN"[(int)s->seq[i]]; - kputc('\t', str); - if (s->qual) { // printf qual - ks_resize(str, str->l + (qe - qb) + 1); - for (i = qe-1; i >= qb; --i) str->s[str->l++] = s->qual[i]; - str->s[str->l] = 0; - } else kputc('*', str); - } - if (NM >= 0) { kputsn("\tNM:i:", 6, str); kputw(NM, str); } - if (p->score >= 0) { kputsn("\tAS:i:", 6, str); kputw(p->score, str); } - if (p->sub >= 0) { kputsn("\tXS:i:", 6, str); kputw(p->sub, str); } - if (bwa_rg_id[0]) { kputsn("\tRG:Z:", 6, str); kputs(bwa_rg_id, str); } - if (s->comment) { kputc('\t', str); kputs(s->comment, str); } - kputc('\n', str); - free(cigar); -#undef is_mapped -} - static inline int get_rlen(int n_cigar, const uint32_t *cigar) { int k, l; @@ -834,46 +727,6 @@ int mem_approx_mapq_se(const mem_opt_t *opt, const mem_alnreg_t *a) return mapq; } -void mem_alnreg2hit(const mem_alnreg_t *a, bwahit_t *h) -{ - h->rb = a->rb; h->re = a->re; h->qb = a->qb; h->qe = a->qe; - h->score = a->score; - h->sub = a->secondary >= 0? -1 : a->sub > a->csub? a->sub : a->csub; - h->qual = 0; // quality unset - h->flag = a->secondary >= 0? 0x100 : 0; // only the "secondary" bit is set -} - -void mem_sam_se(const mem_opt_t *opt, const bntseq_t *bns, const uint8_t *pac, bseq1_t *s, mem_alnreg_v *a, int extra_flag, const bwahit_t *m) -{ - int k; - kstring_t str; - str.l = str.m = 0; str.s = 0; - if (a->n > 0 && a->a[0].score >= opt->T) { - int mapq0 = -1; - for (k = 0; k < a->n; ++k) { - bwahit_t h; - mem_alnreg_t *p = &a->a[k]; - if (p->score < opt->T) continue; - if (p->secondary >= 0 && !(opt->flag&MEM_F_ALL)) continue; - if (p->secondary >= 0 && p->score < a->a[p->secondary].score * .5) continue; - mem_alnreg2hit(p, &h); - bwa_fix_xref(opt->mat, opt->q, opt->r, opt->w, bns, pac, (uint8_t*)s->seq, &h.qb, &h.qe, &h.rb, &h.re); - h.flag |= extra_flag; - if ((opt->flag&MEM_F_NO_MULTI) && k && p->secondary < 0) h.flag |= 0x10000; // print the sequence, but flag as secondary (for Picard) - h.qual = p->secondary >= 0? 0 : mem_approx_mapq_se(opt, p); - if (k == 0) mapq0 = h.qual; - else if (h.qual > mapq0) h.qual = mapq0; - bwa_hit2sam(&str, opt->mat, opt->q, opt->r, p->w, bns, pac, s, &h, opt->flag&MEM_F_HARDCLIP, m); - } - } else { - bwahit_t h; - memset(&h, 0, sizeof(bwahit_t)); - h.rb = h.re = -1; h.flag = extra_flag; - bwa_hit2sam(&str, opt->mat, opt->q, opt->r, opt->w, bns, pac, s, &h, opt->flag&MEM_F_HARDCLIP, m); - } - s->sam = str.s; -} - void mem_reg2sam_se(const mem_opt_t *opt, const bntseq_t *bns, const uint8_t *pac, bseq1_t *s, mem_alnreg_v *a, int extra_flag, const mem_aln_t *m) { kstring_t str; @@ -1024,7 +877,6 @@ static void *worker2(void *data) if (!(w->opt->flag&MEM_F_PE)) { for (i = w->start; i < w->n; i += w->step) { mem_mark_primary_se(w->opt, w->regs[i].n, w->regs[i].a); - //mem_sam_se(w->opt, w->bns, w->pac, &w->seqs[i], &w->regs[i], 0, 0); mem_reg2sam_se(w->opt, w->bns, w->pac, &w->seqs[i], &w->regs[i], 0, 0); free(w->regs[i].a); } diff --git a/bwamem.h b/bwamem.h index 55cff76f..ae201e6a 100644 --- a/bwamem.h +++ b/bwamem.h @@ -59,13 +59,6 @@ typedef struct { double avg, std; } mem_pestat_t; -typedef struct { // TODO: This is an intermediate struct only. Better get rid of it. - int64_t rb, re; - int qb, qe, flag, qual; - // optional info - int score, sub; -} bwahit_t; - typedef struct { // This struct is only used for the convenience of API. int64_t pos; // forward strand 5'-end mapping position int rid; // reference sequence index in bntseq_t; <0 for unmapped diff --git a/bwamem_pair.c b/bwamem_pair.c index 7cbf5e03..5f2c7bb9 100644 --- a/bwamem_pair.c +++ b/bwamem_pair.c @@ -169,7 +169,6 @@ int mem_matesw(const mem_opt_t *opt, int64_t l_pac, const uint8_t *pac, const me int mem_pair(const mem_opt_t *opt, int64_t l_pac, const uint8_t *pac, const mem_pestat_t pes[4], bseq1_t s[2], mem_alnreg_v a[2], int id, int *sub, int *n_sub, int z[2]) { - extern void mem_alnreg2hit(const mem_alnreg_t *a, bwahit_t *h); pair64_v v, u; int r, i, k, y[4], ret; // y[] keeps the last hit kv_init(v); kv_init(u);