git clone https://github.com/CadenZhao/omics-pipeline.git
cd omics-pipeline/omxpipeline
# use -h flag to check the usage
./SRAdownload-pipeline.py -h
./bulkRNA-pipeline.sh -h
<path to pipeline> -h
easily download fastq file from SRA database requirements:
- sra-toolkit (should include fasterq-dump)
- SraRunTable.txt (downloaded from SRA database)
QC, alignment, gene count, gene TPM, BAM to BigWig
gMode:
trimmomatic
fastqc
multiqc
STAR
samtools
R (with GenomicFeatures package)
tMode:
trimmomatic
fastqc
multiqc
salmon
working on...
working on...
working on...