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Genome and transcriptome bioinformatics analyses pipelines of QX-infected oyster tissues

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Background

Marteilia sydneyi is a protozoan parasite and the causative agent of Queensland Unknown (QX) disease in Saccostrea glomerata (Sydney rock oyster). Disease outbreaks often lead to high mortality rates and considerable economic losses in the aquaculture industry. In order to develop effective disease management and breed strategies, it is imperative to understand the host/parasite interaction at a molecular level. This study aims to investigate S. glomerata’s response to M. sydneyi infection through differential gene expression analysis to uncover immune mechanisms and potential markers for resistance.

This repository contains the bioinformatics pipeline used to perform the following research aims:

Aims

  • Genomic and transcriptomic characterisation of Marteilia sydneyi
  • Identify genes that play a role in the defence response of Sydney Rock Oysters (Saccostrea glomerata) during infection of Marteilia sydneyi (QX disease)

Objectives

  1. Assemble the Marteilia sydneyi transcriptome
  2. Identify differential gene expression in infected and non-infected Sydney Rock Oysters (Saccostrea glomerata)
  3. Assemble the Marteilia sydneyi genome

Detailed information on the experimental and analysis approaches and methods, including detailed bioinformatics pipelines and code can be found at https:/idobar.github.io/QX_bioinfo_analysis/index.html.

This repository was created by Ido Bar ([email protected]) and Niki Nenadic ([email protected]) in collaboration with Carmel McDougall ([email protected]).
Please contact us for any additional information or collaboration opportunities.

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Genome and transcriptome bioinformatics analyses pipelines of QX-infected oyster tissues

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