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aseedb committed Jan 24, 2023
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Expand Up @@ -17,32 +17,32 @@ Data information: (A, C, E) Synapses in mouse cultured neurons. (B, D, F) Rat sy
![**Tethered connected SVs**
(A, B) Tomographic slices showing tethered connected vesicles. Blue arrows highlight the connectors. Scale bar, 50 nm.](images/supplementary_connected_vesicles.png){#fig:suppl_connected_slices width="8cm" tag="EV4"}

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| ID | Exocytosis stage | Vesicles per tomogram | Tethers per AZ | AZ surface area [µm²] | Connectors per synapse (0-250 nm) |
|:----------------------------------------:|:----------------:|:---------------------:|:--------------:|:---------------------:|:----------------------------------:|
| Control 1 | not stimulated | 220 | 15 | 0.11 | 331 |
| Control 2 | not stimulated | 104 | 8 | 0.04 | 264 |
| Control 3 | not stimulated | 127 | 4 | 0.05 | 230 |
| Control 4 | not stimulated | 143 | 3 | 0.03 | 482 |
| Control 5 | not stimulated | 213 | 12 | 0.11 | 361 |
| Control 6 | not stimulated | 104 | 7 | 0.06 | 199 |
| Control 7 | not stimulated | 184 | 9 | 0.03 | 360 |
| Control 8 | not stimulated | 132 | 19 | 0.09 | 226 |
| Control 9 | not stimulated | 134 | 6 | 0.08 | 326 |
| Spray 1 | late | 697 | 5 | 0.03 | 272 |
| Spray 2 | late | 115 | 3 | 0.08 | 88 |
| Spray 3 | late | 429 | 21 | 0.19 | 882 |
| Spray 5 | early | 534 | 57 | 0.07 | 1412 |
| Spray 5_2 (second AZ, same synaptosome) | early | | 32 | 0.15 | |
| Spray 6 | late | 371 | 1 | 0.03 | 397 |
| Spray 7 | early | 107 | 5 | 0.02 | 156 |
| Spray 8 | late | 99 | 4 | 0.02 | 202 |
| Spray 10 | late | 76 | 4 | 0.02 | 96 |
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| ID | Exocytosis stage | Vesicles per tomogram | Tethers per AZ | AZ surface area [µm²] | Connectors per synapse (0-250 nm) |
|:----------------------------------------:|:-----------------:|:---------------------:|:--------------:|:---------------------:|:---------------------------------:|
| Control 1 | not stimulated | 220 | 15 | 0.11 | 331 |
| Control 2 | not stimulated | 104 | 8 | 0.04 | 264 |
| Control 3 | not stimulated | 127 | 4 | 0.05 | 230 |
| Control 4 | not stimulated | 143 | 3 | 0.03 | 482 |
| Control 5 | not stimulated | 213 | 12 | 0.11 | 361 |
| Control 6 | not stimulated | 104 | 7 | 0.06 | 199 |
| Control 7 | not stimulated | 184 | 9 | 0.03 | 360 |
| Control 8 | not stimulated | 132 | 19 | 0.09 | 226 |
| Control 9 | not stimulated | 134 | 6 | 0.08 | 326 |
| Spray 1 | late | 697 | 5 | 0.03 | 272 |
| Spray 2 | late | 115 | 3 | 0.08 | 88 |
| Spray 3 | late | 429 | 21 | 0.19 | 882 |
| Spray 5 | early | 534 | 57 | 0.07 | 1412 |
| Spray 5_2 (second AZ, same synaptosome) | early | | 32 | 0.15 | |
| Spray 6 | late | 371 | 1 | 0.03 | 397 |
| Spray 7 | early | 107 | 5 | 0.02 | 156 |
| Spray 8 | late | 99 | 4 | 0.02 | 202 |
| Spray 10 | late | 76 | 4 | 0.02 | 96 |
Table: Summary of the synaptosome tomograms.
{#tbl:synaptosome-tomograms tag="EV1"}

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| ID | Mutation | Vesicles per Tomogram | Tethers per AZ | AZ surface area [µm²] | Connectors per synapse (0-250 nm) |
|:---:|:--------:|:----------------------:|:--------------:|:---------------------:|:---------------------------------:|
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