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Make Complex Heatmaps

R-CMD-check codecov bioc bioc

Complex heatmaps are efficient to visualize associations between different sources of data sets and reveal potential patterns. Here the ComplexHeatmap package provides a highly flexible way to arrange multiple heatmaps and supports various annotation graphics.

The InteractiveComplexHeatmap package can directly export static complex heatmaps into an interactive Shiny app. Have a try!

Citation

Zuguang Gu, et al., Complex heatmaps reveal patterns and correlations in multidimensional genomic data, Bioinformatics, 2016

Install

ComplexHeatmap is available on Bioconductor, you can install it by:

if (!requireNamespace("BiocManager", quietly=TRUE))
    install.packages("BiocManager")
BiocManager::install("ComplexHeatmap")

If you want the latest version, install it directly from GitHub:

library(devtools)
install_github("jokergoo/ComplexHeatmap")

Usage

Make a single heatmap:

Heatmap(mat, ...)

A single Heatmap with column annotations:

ha = HeatmapAnnotation(df = anno1, anno_fun = anno2, ...)
Heatmap(mat, ..., top_annotation = ha)

Make a list of heatmaps:

Heatmap(mat1, ...) + Heatmap(mat2, ...)

Make a list of heatmaps and row annotations:

ha = HeatmapAnnotation(df = anno1, anno_fun = anno2, ..., which = "row")
Heatmap(mat1, ...) + Heatmap(mat2, ...) + ha

Documentation

The full documentations are available at https://jokergoo.github.io/ComplexHeatmap-reference/book/ and the website is at https://jokergoo.github.io/ComplexHeatmap.

Blog posts

There are following blog posts focusing on specific topics:

Examples

Visualize Methylation Profile with Complex Annotations

complexheatmap_example4

Correlations between methylation, expression and other genomic features

complexheatmap_example3

Visualize Cell Heterogeneity from Single Cell RNASeq

complexheatmap_example2

Making Enhanced OncoPrint

complexheatmap_example1

UpSet plot

3D heatmap

image

License

MIT @ Zuguang Gu

Packages

No packages published

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