This is the repository accompanying the Manuscript Single-cell multi-omic detection of DNA methylation and histone modifications reconstructs the dynamics of epigenetic maintenance. It contains links to all the (raw) sequencing data, FACS index data as well as information about the preprocessing and downstream analyses.
Raw data for combined methylation and histone profiling have been deposited at the Gene Expression Omnibus (accession GSE232637).
Raw WGBS data have been deposited at the Gene Expression Omnibus (accession GSE232637).
Data were downloaded from ENCODE:
Count tables for single-cell sortChIC data from Zeller et al. were downloaded from GEO repository GSE164779.
Preprocessing of fastq.gz files was performed using the Package SingleCellMultiOmics
developed by Buys de Barbanson. The software package is available on github.
All code to produce the figures in the manuscript can be found in the subdirectory code
.
Supplementary files containing all adapter and oligo sequences used for sequencing library preparation are summarized in three supplementary tables in the subdirectory tables
.
FACS plots for the experiments in RPE-1 hTERT Fucci cells where deposited in the subdirectory facs_plots
. The subfolder index_data
contains FACS parameters for every single cell, file names refer to the data available via GEO.