Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Can't progress Imputation for Chr X #7

Open
innerpyg-bi opened this issue May 15, 2020 · 7 comments
Open

Can't progress Imputation for Chr X #7

innerpyg-bi opened this issue May 15, 2020 · 7 comments

Comments

@innerpyg-bi
Copy link

I analyzed by following steps.

1. docker run -d -p 4000 genepi/imputationserver:v1.2.7
2. access http://localhost:4000
3. install 1000genomes-phase3 reference panel in Admin Panel

  • But There is no Chr X Reference data. So,

4. local download and make m3vcf and bcf files for Chr X
5. Insert the m3vcf and bcf file into /data/apps/1000g-phase-3-v5/2.0.0 on genepi/imputationserver:v1.2.7
6. docker exec -t -i ImputationServer cloudgene run [email protected] --files /mnt/$PWD/nonPAR.vcf.gz --refpanel apps@[email protected] --conf /etc/hadoop/conf --population eas &> docker.log


In this case, I can see this log.
MI_nonPAR_Error

Please let me know how to fix
Thanks

@seppinho
Copy link
Member

Can you try the same file on https://imputationserver.sph.umich.edu?

@innerpyg-bi
Copy link
Author

Today, I analyzed web based imputation server using same file.
Chr22 vcf file was finished. but not ChrX.

@seppinho
Copy link
Member

thanks for testing. any chances you can share a test file that fails?

@innerpyg-bi
Copy link
Author

I'm sending you a vcf file with only the sample ID changed.
nonPAR_Update.vcf.gz

Thank you for your concern.

@seppinho
Copy link
Member

Had to unzip/bgzip it again but worked then on the server. Can you please double check?
nonPAR_Update.vcf.gz

@innerpyg-bi
Copy link
Author

innerpyg-bi commented May 19, 2020

Oh, i found when doesn't work on web.
When i select the reference panel which is EUR and Other/Mixed poputation of 1000G Phase3 V5 hg19, it works but not the others.
I also checked on docker, but still doesn't work including eur.

@o147942
Copy link

o147942 commented Jun 24, 2020

I cannot find the bcf file of the chrX in bcfs folder in hapmap and 1000g reference panel of docker.
Why there is no chrX file?

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

3 participants