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finna-be-octo-bear

GATK Workflow

  • custom gatk workflow adopted from CIDR
  • Modify to suit the setting at local servers
  1. Install bwa, picard, and gatk in $HOME/bin
  2. Download gatk_bundle files from GATK ftp sites in $GATK_RESOURCE_PATH
    • this includes reference genome (hg19), known indels, dbsnp138
  3. set up bwa index
  4. put fastq files in $PROJECT_FOLDER/$FASTQ_DIR
  5. This script will create a subscript for each process in $SAMPLE/$SCRIPT
  6. Change the number of threads to reflect your CPUs
  7. Change the INPUT DATA below to match with your input

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