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bredeson authored Oct 24, 2019
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11. Use `samtools view` to keep alignments with `PAIRED` and `PROPER_PAIR` flags *AND DO NOT* contain `UNMAP`, `MUNMAP`, `SECONDARY`, `QCFAIL`, `DUP`, or `SUPPLEMENTARY` flags; write the output as a BAM file. Index this new file with SAMtools (*HINT*: see `samtools flags` for help with flags). Be sure to index your filtered BAM file.

12. Using the base quality score determined in *Step 9* as the minimum base quality threshold, call SNPs using the GATK HaplotypeCaller.
12. Using the base quality score determined in *Step 10* as the minimum base quality threshold, call SNPs using the GATK HaplotypeCaller.

13. Use the `samtools depth` command to calculate the per-site depth of reads in the genome (see `samtools depth --help` for more info). The output file contains three columns: the chromosome name, position (1-based), and depth. For example:
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