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Update README.md
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vdemichev authored Jul 15, 2024
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Expand Up @@ -201,7 +201,7 @@ A text table containing precursor and protein IDs, as well as plenty of associat
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<summary>Matrices</summary>

These contain normalised MaxLFQ quantities for protein groups ('pg_matrix'), gene groups ('gg_matrix'), unique genes ('unique_genes_matrix'; i.e. genes identified and quantified using only proteotypic, that is gene-specific, peptides) as well as normalised quantities for precursors ('pr_matrix'). They are filtered at 1% FDR, using global q-values for protein groups and both global and run-specific q-values for precursors. Additional 5% run-specific protein-level FDR filter is applied to the protein matrices, use --matrix-spec-q to adjust it. Sometimes DIA-NN will report a zero as the best estimate for a precursor or protein quantity. Such zero quantities are omitted from protein/gene matrices.
These contain normalised MaxLFQ quantities for protein groups ('pg_matrix'), gene groups ('gg_matrix'), unique genes ('unique_genes_matrix'; i.e. genes identified and quantified using only proteotypic, that is gene-specific, peptides) as well as normalised quantities for precursors ('pr_matrix'). They are filtered at 1% FDR, using global q-values for protein groups and both global and run-specific q-values for precursors. Additional 5% run-specific protein-level FDR filter is applied to the protein matrices, use --matrix-spec-q to adjust it. Sometimes DIA-NN will report a zero as the best estimate for a precursor or protein quantity. Such zero quantities are omitted from protein/gene matrices. Special phosphosite quantification matrices (phosphosites_90 and phosphosites_99 .tsv) are generated when phosphorylation (UniMod:21) is declared as a variable modification, see [PTMs and peptidoforms](#ptms-and-peptidoforms).
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