Merge pull request #52 from aryeelab/bugfix/lowercase

Fixed bug where reverse complement off-targets are missed in lower case reference. Fixes #51
aryeelab · Jun 1, 2019 · c608522 · c608522
2 parents 6f3c5de + 834862f
commit c608522
Showing 1 changed file with 1 addition and 1 deletion.
diff --git a/guideseq/identifyOfftargetSites.py b/guideseq/identifyOfftargetSites.py
@@ -298,7 +298,7 @@ def processLine(line):
 
 
 def reverseComplement(sequence):
-    transtab = string.maketrans("ACGT", "TGCA")
+    transtab = string.maketrans("ACGTacgt", "TGCATGCA")
     return sequence.translate(transtab)[::-1]