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// To compile: | ||
// gcc -g -O2 example.c libminimap2.a -lz | ||
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#include <stdlib.h> | ||
#include <assert.h> | ||
#include <stdio.h> | ||
#include <zlib.h> | ||
#include "minimap.h" | ||
#include "kseq.h" | ||
KSEQ_INIT(gzFile, gzread) | ||
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int main(int argc, char *argv[]) | ||
{ | ||
mm_verbose = 2; // disable message output to stderr | ||
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if (argc < 3) { | ||
fprintf(stderr, "Usage: minimap2-lite <target.fa> <query.fa>\n"); | ||
return 1; | ||
} | ||
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// open query file for reading; you may use your favorite FASTA/Q parser | ||
gzFile f = gzopen(argv[2], "r"); | ||
assert(f); | ||
kseq_t *ks = kseq_init(f); | ||
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// create index for target; we are creating one index for all target sequence | ||
int n_threads = 4, w = 10, k = 15, is_hpc = 0; | ||
mm_idx_t *mi = mm_idx_build(argv[1], w, k, is_hpc, n_threads); | ||
assert(mi); | ||
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// mapping | ||
mm_mapopt_t opt; | ||
mm_mapopt_init(&opt); // initialize mapping parameters | ||
mm_mapopt_update(&opt, mi); // this sets the maximum minimizer occurrence; TODO: set a better default in mm_mapopt_init()! | ||
opt.flag |= MM_F_CIGAR; // perform alignment | ||
mm_tbuf_t *tbuf = mm_tbuf_init(); // thread buffer; for multi-threading, allocate one tbuf for each thread | ||
while (kseq_read(ks) >= 0) { // each kseq_read() call reads one query sequence | ||
const mm_reg1_t *reg; | ||
int j, i, n_reg; | ||
// get all hits for the query | ||
reg = mm_map(mi, ks->seq.l, ks->seq.s, &n_reg, tbuf, &opt, 0); | ||
// traverse hits and print them out | ||
for (j = 0; j < n_reg; ++j) { | ||
const mm_reg1_t *r = ®[j]; | ||
assert(r->p); // with MM_F_CIGAR, this should not be NULL | ||
printf("%s\t%d\t%d\t%d\t%c\t", ks->name.s, ks->seq.l, r->qs, r->qe, "+-"[r->rev]); | ||
printf("%s\t%d\t%d\t%d\t%d\t%d\t%d\tcg:Z:", mi->seq[r->rid].name, mi->seq[r->rid].len, r->rs, r->re, | ||
r->p->blen - r->p->n_ambi - r->p->n_diff, r->p->blen, r->mapq); | ||
for (i = 0; i < r->p->n_cigar; ++i) // IMPORTANT: this gives the CIGAR in the aligned regions. NO soft/hard clippings! | ||
printf("%d%c", r->p->cigar[i]>>4, "MIDSHN"[r->p->cigar[i]&0xf]); | ||
putchar('\n'); | ||
} | ||
} | ||
mm_tbuf_destroy(tbuf); | ||
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// deallocate index and close the query file | ||
mm_idx_destroy(mi); | ||
kseq_destroy(ks); | ||
gzclose(f); | ||
return 0; | ||
} |